Analysis Note

ControlPOSITIVE CONTROL: Jurkat cells

Application

ELISA: 1:10,000 (direct)

Western blot: 1:1,000 to 1:5,000. On western blots of Jurkat cell extracts the antibody recognizes a major band at 140 kDa corresponding to Integrin alpha4 as well as 3 weak bands at approximately 60 kDa that are believed to be non-specific.

Immunoprecipitation: 5 µL per 500 µg whole Jurkat cell lysate.

NOTE: the use of specific inhibitors of phosphoserine phosphatse activity such as okadaic acid or NaF is strongly recommended during the preparation of protein extracts for western blot and immunoprecipitation.

Optimal working dilutions must be determined by end user.

This Anti-Integrin α4 Antibody, PhosphoSerine 988 is validated for use in ELISA, IP, WB for the detection of Integrin α4.

Research Sub CategoryIntegrins

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Integrin alpha4 has been implicated in the pathogenesis of diseases such as asthma, arthritis, and multiple sclerosis. Phosphorylation of the cytosolic tail of integrin alpha4 at position 988 is required for optimal alpha4beta1 integrin-mediated directed cell migration. In the absence of phosphorylation at Ser988, the cytosolic tail of integrin alpha4 binds tightly to the signaling adaptor protein, paxillin, inhibiting cell migration. Phosphorylation of alpha4 at Ser988 promotes dissociation from paxillin thereby enhancing cell migration (Han, 2003). MW is approximately 150 kDa.

Immunogen

Synthetic peptide corresponding to the cytoplasmic tail of human integrin alpha4 (a.a. 986-995) containing a phosphorylated serine at position 988. The phosphopeptide was conjugated to KLH using gluteraldehyde.

Epitope: PhosphoSerine 988

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid rabbit serum containing no preservative.

Specificity

Integrin alpha4 phosphorylated on serine 988. Does not react with integrin alpha4 that is not phosphorylated at serine 988 (Han, 2001).

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.